audit_missing_seqspec

audit_no_files

audit_missing_auxiliary_set

Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

CRISPR FACS screen

guide (sgRNA) library targeting transcription start sites in IL2RA

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA

Accession

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Assay Term

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Functional Assay Mechanisms

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MeasurementSet

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Auxiliary Data

Construct Library Data

Investigated Gene

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Audit Category: Internal Action

Externally Hosted

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