audit_missing_seqspec

Jay Shendure

Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems

Jay Shendure, UW

scCRISPR screen

guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

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Audit Category: Internal Action

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