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Assay
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in vitro CRISPR screen using single-cell RNA-seq
1
Preferred Assay Title
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scCRISPR screen
1
Auxiliary Data
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gRNA sequencing
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Construct Library Data
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guide library
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Sequencing Platform
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Illumina NextSeq 2000
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auxiliary_sets.@id
/auxiliary-sets/IGVFDS4502LQQH/
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MeasurementSet
IGVFDS4863BMGZ
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Jay Shendure, UW
Sample Summary
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
released