audit_missing_seqspec

audit_no_files

audit_missing_auxiliary_set

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Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

CRISPR FACS screen

guide (sgRNA) library targeting transcription start sites in B2M

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M

Accession

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Assay Term

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Functional Assay Mechanisms

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UUID

MeasurementSet

Measurement Set Details

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Auxiliary Data

Construct Library Data

Investigated Gene

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Audit Category: Internal Action

Externally Hosted

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