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Assay
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in vitro CRISPR screen using single-cell RNA-seq
1
Preferred Assay Title
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TAP-seq
1
Auxiliary Data
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gRNA sequencing
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Construct Library Data
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guide library
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Illumina NextSeq 550
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auxiliary_sets.@id
/auxiliary-sets/IGVFDS0282GEHF/
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MeasurementSet
IGVFDS3171OFHP
mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci
Jesse Engreitz, Stanford
Sample Summary
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released