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Jesse Engreitz

Stanford Center for Connecting DNA Variants to Function and Phenotype

Jesse Engreitz, Stanford

CRISPR FlowFISH screen

guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3

TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3

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