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IGVFFI9036JJXB

released
validated
File Set
IGVFDS3778BYII(Homo sapiens GRCh38 custom guide RNAs)
Summary
custom assembly elements reference
File Format
fasta
Content Type
elements reference
Aliases
charles-gersbach:cd8-tcells-B2M-crispr-screen-grnas-fasta
md5sum
5b722a2a99bfb396c2edcc023fb8ed4d
Content MD5sum
8946bb7e0641012a6cf0797245c1eebe
File Size
4.3 KB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/CRISPRi.B2M.grna.fa.gz

Reference Source Details

Genome Assembly
custom

Samples

6 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke