IGVFFI9036JJXB

released
validated
{
    "@context": "/terms/",
    "@id": "/reference-files/IGVFFI9036JJXB/",
    "@type": [
        "ReferenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI9036JJXB",
    "aliases": [
        "charles-gersbach:cd8-tcells-B2M-crispr-screen-grnas-fasta"
    ],
    "assay_titles": [],
    "assembly": "custom",
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released reference file [IGVFFI9036JJXB](/reference-files/IGVFFI9036JJXB/) has in progress subobject curated set [IGVFDS3778BYII](/curated-sets/IGVFDS3778BYII/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/reference-files/IGVFFI9036JJXB/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "8946bb7e0641012a6cf0797245c1eebe",
    "content_type": "elements reference",
    "controlled_access": false,
    "creation_timestamp": "2024-10-15T18:31:28.792706+00:00",
    "external": false,
    "file_format": "fasta",
    "file_set": {
        "@id": "/curated-sets/IGVFDS3778BYII/",
        "accession": "IGVFDS3778BYII",
        "file_set_type": "guide RNAs",
        "samples": [
            {
                "@id": "/primary-cells/IGVFSM3427LEEW/",
                "accession": "IGVFSM3427LEEW",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM1190UUKW/",
                "accession": "IGVFSM1190UUKW",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM4508IIAY/",
                "accession": "IGVFSM4508IIAY",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM7452ZWAU/",
                "accession": "IGVFSM7452ZWAU",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM0015KLZS/",
                "accession": "IGVFSM0015KLZS",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM2690MTOA/",
                "accession": "IGVFSM2690MTOA",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "in progress",
        "summary": "Homo sapiens GRCh38 custom guide RNAs",
        "taxa": "Homo sapiens"
    },
    "file_size": 4333,
    "gene_list_for": [],
    "href": "/reference-files/IGVFFI9036JJXB/@@download/IGVFFI9036JJXB.fasta.gz",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "md5sum": "5b722a2a99bfb396c2edcc023fb8ed4d",
    "release_timestamp": "2024-12-16T18:54:58.899309+00:00",
    "s3_uri": "s3://igvf-files/2024/10/15/4edb50f4-f6ed-4c44-8390-e51fde1306d6/IGVFFI9036JJXB.fasta.gz",
    "schema_version": "15",
    "status": "released",
    "submitted_by": {
        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
        "title": "Alejandro Barrera"
    },
    "submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/CRISPRi.B2M.grna.fa.gz",
    "summary": "custom assembly elements reference",
    "upload_status": "validated",
    "uuid": "4edb50f4-f6ed-4c44-8390-e51fde1306d6"
}