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CD8-positive, alpha-beta memory T cell — IGVFSM8084QKPW

released
Summary
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)
Sample Terms
Sex
unspecified
Age
unknown
Construct Library Sets
Taxa
Homo sapiens
Sources
stem-cell-technologies
Sorted From Sample
IGVFSM2824DRGN FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3
Aliases
charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-high-bin-rep1
Publications
Attribution
Charles Gersbach, Duke

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-25
unspecified
Homo sapiens

File Sets

3 items
CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes
  • charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-batf3-over-high-bin-rep1
Charles Gersbach, Duke
gRNA sequencing
  • charles-gersbach:cd8-tcells-auxiliary-set-crispr-ko-tfome-batf3-over-high-bin-rep1
Charles Gersbach, Duke
CRISPR knockout FACS screen targeting IL7R: alignments, fold change over control, guide quantifications
  • charles-gersbach:cd8-tcells-analysis-set-crispr-ko-tfome-batf3-over
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
Activation
knockout