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CD8-positive, alpha-beta memory T cell — IGVFSM7887TFLH

released
Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Sex
unspecified
Age
unknown
Taxa
Homo sapiens
Sources
stem-cell-technologies
Sorted From Sample
IGVFSM2082DWDD FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3
Aliases
charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-batf3-over-high-bin-rep2
Publications

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-26
unspecified
Homo sapiens

File Sets

5 items
gRNA sequencing for CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
  • charles-gersbach:cd8-tcells-auxiliary-set-crispr-ko-tfome-batf3-over-high-bin-rep2
Charles Gersbach, Duke
CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
  • charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-batf3-over-high-bin-rep2
Charles Gersbach, Duke
CRISPR knockout FACS screen targeting IL7R: alignments
  • charles-gersbach:cd8-tcells-intermediate-analysis-crispr-ko-tfome-batf3-over
Charles Gersbach, Duke
CRISPR knockout FACS screen targeting IL7R: fold change over control, guide quantifications
  • charles-gersbach:cd8-tcells-analysis-set-crispr-ko-tfome-batf3-over
Charles Gersbach, Duke
Homo sapiens GRCh38 custom guide RNAs
  • charles-gersbach:tfome-grnas-set
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
knockout

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke