IGVFDS6902RBPO

in vitro CRISPR screen using flow cytometry

experimental data

CRISPR FACS screen

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

released

CRISPR interference FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes

IGVFDS5825GTXD

gRNA sequencing

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

revoked

gRNA sequencing

IGVFDS9143TFRW

/human-donors/IGVFDO3231VPAA/, /human-donors/IGVFDO5479ZYLJ/

principal analysis

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4), Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

/primary-cells/IGVFSM7413JCSK/, /primary-cells/IGVFSM1205VMNO/, /primary-cells/IGVFSM9398LTWW/, /primary-cells/IGVFSM2207SJEN/

Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRi Sa-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of CCR7

released

CRISPR interference FACS screen targeting CCR7: alignments, fold change over control, guide quantifications