IGVFDS4215TFWE

gRNA sequencing

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

revoked

gRNA sequencing

IGVFDS7858OVUD

in vitro CRISPR screen using flow cytometry

experimental data

CRISPR FACS screen

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

released

CRISPR knockout FACS screen sorted on the expression of IL7R integrating a guide (sgRNA) library targeting transcription start sites in many genes

IGVFDS5106XTZH

/human-donors/IGVFDO6692QWLN/, /human-donors/IGVFDO4646JDEN/

principal analysis

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4), Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

/primary-cells/IGVFSM2624KTRK/, /primary-cells/IGVFSM4936HKEW/, /primary-cells/IGVFSM7226CVDS/, /primary-cells/IGVFSM0466RHGQ/

Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRko Sp-Cas9, transfected with a guide library, sorted on expression of IL7R

released

CRISPR knockout FACS screen targeting IL7R: alignments, fold change over control, guide quantifications