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samples.@id
/primary-cells/IGVFSM6449HHZS/
2 items
IGVFDS8404CSTN
  • charles-gersbach:hPA-measurement-set-perturbseq-10xlane2-GEX
in vitro CRISPR screen using single-cell RNA-seq
no certificate
reads, seqspec
experimental data
Perturb-seq
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library
released
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes
IGVFDS1645YFQD
  • charles-gersbach:hPA-auxiliary-set-perturbseq-10xlane2-CRISPR
no certificate
reads, seqspec
gRNA sequencing
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library
released
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes