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samples.@id
/primary-cells/IGVFSM6223YCQV/
3 items
IGVFDS1675WFHV
  • charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep2
in vitro CRISPR screen using single-cell RNA-seq
experimental data
Perturb-seq
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
released
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
IGVFDS0993OMZM
  • charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep2
gRNA sequencing
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
released
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
IGVFDS5239SBBT
  • charles-gersbach:cd8-tcells-analysis-set-perturb-seq-crispra
principal analysis
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRa Sa-dCas9-VP64, transfected with a guide library
released
activation Perturb-seq: differential gene expression quantifications, filtered feature barcode matrix