IGVFDS5357XHOF

in vitro CRISPR screen using flow cytometry

experimental data

CRISPR FACS screen

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

released

low FACS signal CRISPR interference FACS screen sorted on the expression of B2M integrating a guide (sgRNA) library targeting transcription start sites in B2M

IGVFDS8839AFQB

/human-donors/IGVFDO4200TDAP/, /human-donors/IGVFDO3455VIHN/, /human-donors/IGVFDO8199EHWU/

principal analysis

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4), Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

/primary-cells/IGVFSM7452ZWAU/, /primary-cells/IGVFSM6062UNTJ/, /primary-cells/IGVFSM4508IIAY/, /primary-cells/IGVFSM1190UUKW/, /primary-cells/IGVFSM3427LEEW/, /primary-cells/IGVFSM3073WHFV/

Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRi Sa-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of B2M

released

CRISPR interference FACS screen targeting B2M: alignments, fold change over control, guide quantifications

IGVFDS9580JWWG

gRNA sequencing

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

revoked

gRNA sequencing