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samples.@id
/primary-cells/IGVFSM2747RJFY/
3 items
IGVFDS2399UCGA
  • charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep2
gRNA sequencing
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
released
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
IGVFDS9445RJOU
  • charles-gersbach:cd8-tcells-analysis-set-perturb-seq-crispri
principal analysis
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRi Sa-dCas9-KOX1-KRAB, transfected with a guide library
released
interference Perturb-seq: differential gene expression quantifications, filtered feature barcode matrix
IGVFDS1333MBPK
  • charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep2
in vitro CRISPR screen using single-cell RNA-seq
experimental data
Perturb-seq
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
released
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes