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samples.@id
/primary-cells/IGVFSM2624KTRK/
4 items
IGVFDS0140NIXT
  • charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-low-bin-rep2
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FACS screen
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
released
CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
IGVFDS5106XTZH
  • charles-gersbach:cd8-tcells-analysis-set-crispr-ko-tfome
principal analysis
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4), CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
released
CRISPR knockout FACS screen targeting IL7R: fold change over control, guide quantifications
IGVFDS5551EATG
  • charles-gersbach:cd8-tcells-intermediate-analysis-crispr-ko-tfome
intermediate analysis
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4), CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
released
CRISPR knockout FACS screen targeting IL7R: alignments
IGVFDS3348HSLB
  • charles-gersbach:cd8-tcells-auxiliary-set-crispr-ko-tfome-low-bin-rep2
gRNA sequencing
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
revoked
gRNA sequencing