Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR interference FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes
guide quantifications, fold change over control, alignments
principal analysis
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4), Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
Homo sapiens CD8-positive, alpha-beta memory T primary cell, modified with CRISPRi Sa-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of CCR7
CRISPR interference FACS screen targeting CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes with low FACS signal control