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samples.@id
/in-vitro-systems/IGVFSM8464JMJS/
4 items
IGVFDS4262EBDF
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_NPC_sgRNA_LW283
gRNA sequencing
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW283) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS2600XYHD
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_NPC_HTO_LW283
cell hashing barcode sequencing
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW283) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS1588TVKF
  • gary-hon:WTC11_TF_Perturb_Seq_Pilot_NPC_Lenti_PrimaryAnalysis
principal analysis
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW281) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW283) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW282) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW284) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Homo sapiens GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell, at 20 day(s) post change, modified with CRISPRi Sp-dCas9, transfected with a guide library
preview
interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix
IGVFDS4028IRAH
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_NPC_LW283
in vitro CRISPR screen using single-cell RNA-seq
experimental data
Perturb-seq
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW283) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci