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samples.@id
/in-vitro-systems/IGVFSM8317ZTFV/
3 items
IGVFDS2385QENN
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_exprs
in vitro CRISPR screen using single-cell RNA-seq
experimental data
scCRISPR screen
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
released
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
IGVFDS2532AVSC
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_CS1
gRNA sequencing
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
released
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
IGVFDS4021XJLW
  • jay-shendure:analysis_set_CRISPRa_ipscNeurons
principal analysis
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane4) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
Homo sapiens GM25256 (WTC-11) differentiated cell specimen induced to neuron, at 19 day(s) post change, modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
released
scCRISPR activation screen: differential gene expression quantifications, filtered feature barcode matrix