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3 items
IGVFDS9621FJCZ
  • jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin0hrs-FF1
cell sorting
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
IGVFDS9168ZKDC
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinA-PCR1_S1
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinA-PCR2_S7
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinA-PCR3_S97
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF1-BinA-PCR4_S103
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS3199FLOT
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinA-PCR1_S1
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinA-PCR2_S7
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinA-PCR3_S97
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF1-BinA-PCR4_S103
gRNA sequencing
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
revoked
gRNA sequencing