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samples.@id
/in-vitro-systems/IGVFSM7748IVLZ/
3 items
IGVFDS2261TIMI
  • charles-gersbach:jurkats-analysis-set-crispra-vp64-il2ra
principal analysis
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4), Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Homo sapiens Jurkat cell line, modified with CRISPRa Sa-dCas9-VP64, transfected with a guide library, sorted on expression of IL2RA
released
CRISPR activation FACS screen targeting IL2RA: alignments, fold change over control, guide quantifications
IGVFDS0128XYUT
  • charles-gersbach:jurkats-auxiliary-set-crispra-vp64-il2ra-high-bin-rep3
gRNA sequencing
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
revoked
gRNA sequencing
IGVFDS4959TRIO
  • charles-gersbach:jurkats-measurement-set-crispra-vp64-il2ra-high-bin-rep3
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FACS screen
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
released
CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA