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samples.@id
/in-vitro-systems/IGVFSM6928JPHO/
3 items
IGVFDS9190KTAF
  • jesse-engreitz:auxiliary_sortparams_CRUDO_FAM3C_Auxin6hrs-FF1
cell sorting
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
IGVFDS2315UDUR
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR1-Rep1_S136
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR2-Rep1_S142
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR3-Rep1_S190
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR4-Rep1_S196
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS9525OEUV
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR1-Rep1_S136
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR2-Rep1_S142
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR3-Rep1_S190
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF1-BinD-PCR4-Rep1_S196
gRNA sequencing
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
revoked
gRNA sequencing