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samples.@id
/in-vitro-systems/IGVFSM5744WHTT/
3 items
IGVFDS1500HTAU
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep1_S6
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep2_S61
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep1_S12
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep2_S67
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep1_S18
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep2_S73
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep1_S24
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep2_S79
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS8794YVNF
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep1_S6
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR1_Rep2_S61
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep1_S12
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR2_Rep2_S67
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep1_S18
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR3_Rep2_S73
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep1_S24
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep2_S79
gRNA sequencing
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
revoked
gRNA sequencing
IGVFDS5951VSHM
  • jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin0hrs-FF1
cell sorting
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets