IGVFDS7242NZGL

gRNA sequencing

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

revoked

gRNA sequencing

IGVFDS2046SNMC

in vitro CRISPR screen using flow cytometry

experimental data

CRISPR FlowFISH screen

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

released

mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

IGVFDS5292OCBC

cell sorting

/measurement-sets/IGVFDS2046SNMC/, /measurement-sets/IGVFDS3741DKGN/, /measurement-sets/IGVFDS4315AMOI/, /measurement-sets/IGVFDS1099HASC/, /measurement-sets/IGVFDS8112FQWI/, /measurement-sets/IGVFDS8123YLWP/

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

/in-vitro-systems/IGVFSM7865RIGH/, /in-vitro-systems/IGVFSM7863HHJP/, /in-vitro-systems/IGVFSM5079ZTJX/, /in-vitro-systems/IGVFSM4919VWFX/, /in-vitro-systems/IGVFSM1617PVUY/, /in-vitro-systems/IGVFSM4674ZTIR/

released

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets