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samples.@id
/in-vitro-systems/IGVFSM4418NJET/
4 items
IGVFDS3881UZJT
  • gary-hon:differentited_WTC11_CM_Pilot2_Mini_PB9_sgRNA_LW435
gRNA sequencing
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW435) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
preview
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS7818HFMO
  • gary-hon:differentited_WTC11_CM_Pilot2_Mini_PB9_LW435
in vitro CRISPR screen using single-cell RNA-seq
experimental data
Perturb-seq
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW435) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
preview
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS9289FWZN
  • gary-hon:differentited_WTC11_CM_Pilot2_Mini_PB9_HTO_LW435
cell hashing barcode sequencing
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW435) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
preview
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS4252LFRU
  • gary-hon:WTC11_CM_Pilot2_Mini_PB9_PrimaryAnalysis
principal analysis
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW436) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW438) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW435) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW437) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library
Homo sapiens GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell, at 12 day(s) post change, modified with CRISPRi Sp-dCas9, transfected with a guide library
preview
CRISPR interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix, unfiltered global differential expression, unfiltered local differential expression