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2 items
IGVFDS6780CPBH
  • jesse-engreitz:auxiliary_NOS3_CRISPRi_FF_R2_FFrep2_binD_PCR1_S140
gRNA sequencing
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
revoked
gRNA sequencing
IGVFDS9626CAFF
  • jesse-engreitz:measurement_NOS3_CRISPRi_FF_R2_FFrep2_binD_PCR1_S140
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
released
CRISPR interference FlowFISH screen sorted on the expression of NOS3 integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3