in vitro CRISPR screen using flow cytometry

experimental data

CRISPR FlowFISH screen

Homo sapiens (female) TeloHAEC cell line (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library

CRISPR base editing FlowFISH screen sorted on the expression of NFKBIA integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure

principal analysis

Homo sapiens (female) TeloHAEC cell line (sorting details: FACS 80-100% expression of NFKBIA) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library, Homo sapiens (female) TeloHAEC cell line (sorting details: FACS 20-40% expression of NFKBIA) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library, Homo sapiens (female) TeloHAEC cell line (sorting details: FACS 0-20% expression of NFKBIA) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library, Homo sapiens (female) TeloHAEC cell line (sorting details: FACS 60-80% expression of NFKBIA) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library

Homo sapiens TeloHAEC cell line, modified with CRISPR base editing SpRY-ABE8e, transfected with a guide library, sorted on expression of NFKBIA

CRISPR base editing FlowFISH screen targeting NFKBIA: guide quantifications