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samples.@id
/in-vitro-systems/IGVFSM3954RGXF/
3 items
IGVFDS5560HMQA
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep1_S2
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep2_S57
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep1_S8
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep2_S63
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep2_S69
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep1_S20
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep2_S75
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS5951VSHM
  • jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin0hrs-FF1
cell sorting
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
IGVFDS4701YCEL
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep1_S2
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR1_Rep2_S57
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep1_S8
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR2_Rep2_S63
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep2_S69
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep1_S20
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR4_Rep2_S75
gRNA sequencing
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
revoked
gRNA sequencing