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3 items
IGVFDS9728LNRU
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep1_S111
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep2_S166
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep1_S117
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep2_S172
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep1_S123
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep2_S178
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep1_S129
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep2_S184
gRNA sequencing
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
revoked
gRNA sequencing
IGVFDS7182DGDS
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep1_S111
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR1_Rep2_S166
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep1_S117
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR2_Rep2_S172
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep1_S123
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep2_S178
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep1_S129
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR4_Rep2_S184
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS9055BZJI
  • jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin6hrs-FF1
cell sorting
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets