Click and hold a term momentarily to select items without that term.
3 items
IGVFDS0329USVJ
  • jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin0hrs-FF3
cell sorting
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
IGVFDS6689ALTU
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR1_S25
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR2_S31
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR3_S121
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR4_S127
gRNA sequencing
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
revoked
gRNA sequencing
IGVFDS4813YQOH
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR1_S25
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR2_S31
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR3_S121
  • jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF3-BinA-PCR4_S127
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci