IGVFDS9816UKEE

gRNA sequencing

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

revoked

gRNA sequencing

IGVFDS5464XLTY

cell sorting

/measurement-sets/IGVFDS2241NBBY/, /measurement-sets/IGVFDS0528ZIOC/, /measurement-sets/IGVFDS0483TWVD/, /measurement-sets/IGVFDS6001NZOV/, /measurement-sets/IGVFDS7173YRIO/

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

/in-vitro-systems/IGVFSM9315IOJD/, /in-vitro-systems/IGVFSM4779RXHT/, /in-vitro-systems/IGVFSM2913DQHN/, /in-vitro-systems/IGVFSM6400EVKX/, /in-vitro-systems/IGVFSM7928IVYJ/, /in-vitro-systems/IGVFSM4218VHTT/

released

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 3 more measurement sets

IGVFDS0528ZIOC

in vitro CRISPR screen using flow cytometry

experimental data

CRISPR FlowFISH screen

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

released

mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci