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samples.@id
/in-vitro-systems/IGVFSM2726ORRN/
4 items
IGVFDS1128FXRR
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_iPSC_sgRNA_LW286
gRNA sequencing
Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW286) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS3465KEWH
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_iPSC_HTO_LW286
cell hashing barcode sequencing
Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW286) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS5429CXNB
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_iPSC_LW286
in vitro CRISPR screen using single-cell RNA-seq
experimental data
Perturb-seq
Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW286) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS7340YDHF
  • gary-hon:WTC11_TF_Perturb_Seq_Pilot_iPSC_Lenti_PrimaryAnalysis
principal analysis
Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW288) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW286) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW287) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW289) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Homo sapiens GM25256 (WTC-11) cell line, modified with CRISPRi Sp-dCas9, transfected with a guide library
preview
interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix, unfiltered global differential expression, unfiltered local differential expression