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3 items
IGVFDS9328UUVN
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF2-BinF-PCR1-Rep1_S150
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF2-BinF-PCR2-Rep1_S156
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF2-BinF-PCR3-Rep1_S204
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin6hrs-FF2-BinF-PCR4-Rep1_S210
gRNA sequencing
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
revoked
gRNA sequencing
IGVFDS0422FVNU
  • jesse-engreitz:auxiliary_sortparams_CRUDO_FAM3C_Auxin6hrs-FF2
cell sorting
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 3 more measurement sets
IGVFDS9034AAFA
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF2-BinF-PCR1-Rep1_S150
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF2-BinF-PCR2-Rep1_S156
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF2-BinF-PCR3-Rep1_S204
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin6hrs-FF2-BinF-PCR4-Rep1_S210
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci