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4 items
IGVFDS8839RSIR
  • gary-hon:CM_Pilot_2_Mini_HTO_Lenti_LW272
cell hashing barcode sequencing
Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW272) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS4940ELQK
  • gary-hon:CM_Pilot_2_Mini_Lenti_LW272
in vitro CRISPR screen using single-cell RNA-seq
experimental data
Perturb-seq
Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW272) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS2873EPIU
  • gary-hon:H9_shen_construct_sgRNA_Lenti_PrimaryAnalysis
principal analysis
Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW270) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW272) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW271) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW269) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Homo sapiens H9 differentiated cell specimen induced to cardiac muscle cell, at 12 day(s) post change, modified with CRISPRi Sp-dCas9, transfected with a guide library
preview
interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix, unfiltered global differential expression, unfiltered local differential expression
IGVFDS8726ORVQ
  • gary-hon:CM_Pilot_2_Mini_sgRNA_Lenti_LW272
gRNA sequencing
Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW272) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci