IGVFDS1050IJPU

in vitro CRISPR screen using single-cell RNA-seq

experimental data

Perturb-seq

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW273) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

preview

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFDS5841QQSW

gRNA sequencing

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW273) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

preview

gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFDS7977FNRB

cell hashing barcode sequencing

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW273) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

preview

cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFDS4762GRIW

principal analysis

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW273) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library, Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW275) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library, Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW274) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library, Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW276) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

/in-vitro-systems/IGVFSM1063UQVF/, /in-vitro-systems/IGVFSM9532RAXS/, /in-vitro-systems/IGVFSM5849QIEI/, /in-vitro-systems/IGVFSM6716LOHW/

Homo sapiens H9 differentiated cell specimen induced to cardiac muscle cell, at 12 day(s) post change, modified with CRISPRi Sp-dCas9, transfected with a guide library

preview

interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix