IGVFDS2079MGOR

in vitro CRISPR screen using flow cytometry

experimental data

CRISPR FlowFISH screen

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

released

mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci

IGVFDS5877TZTD

cell sorting

/measurement-sets/IGVFDS0964GIQS/, /measurement-sets/IGVFDS5146ALYO/, /measurement-sets/IGVFDS2079MGOR/, /measurement-sets/IGVFDS8571HTVZ/, /measurement-sets/IGVFDS9594DNWM/, /measurement-sets/IGVFDS3802XLBV/

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

/in-vitro-systems/IGVFSM0561OELB/, /in-vitro-systems/IGVFSM8104CCIE/, /in-vitro-systems/IGVFSM6480FETB/, /in-vitro-systems/IGVFSM7735WAOD/, /in-vitro-systems/IGVFSM9116DXLY/, /in-vitro-systems/IGVFSM0904FNLS/

released

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of CCND1 integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets

IGVFDS2850LCWI

gRNA sequencing

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

revoked

gRNA sequencing