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samples.@id
/in-vitro-systems/IGVFSM0604KDJF/
4 items
IGVFDS5228AHGR
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_HTO_LW280
cell hashing barcode sequencing
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS6861GRJI
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_sgRNA_LW280
gRNA sequencing
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS4852MAUM
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_LW280
in vitro CRISPR screen using single-cell RNA-seq
experimental data
Perturb-seq
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
preview
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS4389OUWU
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_PrimaryAnalysis
principal analysis
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW278) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW277) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library, Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Homo sapiens GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell, at 12 day(s) post change, modified with CRISPRi Sp-dCas9, transfected with a guide library
preview
interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix, unfiltered global differential expression, unfiltered local differential expression