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samples.@id
/in-vitro-systems/IGVFSM0599CFOW/
3 items
IGVFDS5686BCWV
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR1-Rep1_S16
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR2-Rep1_S22
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR3-Rep1_S70
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR4-Rep1_S76
gRNA sequencing
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
revoked
gRNA sequencing
IGVFDS4597CMXX
  • jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin0hrs-FF2
cell sorting
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 3 more measurement sets
IGVFDS5251YUKR
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR1-Rep1_S16
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR2-Rep1_S22
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR3-Rep1_S70
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF2-BinD-PCR4-Rep1_S76
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci