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3 items
IGVFDS1546MEZJ
  • jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin0hrs-FF1
cell sorting
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 3 more measurement sets
IGVFDS6286FOBM
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR1-Rep1_S2
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR2-Rep1_S8
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR3-Rep1_S56
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR4-Rep1_S62
gRNA sequencing
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
revoked
gRNA sequencing
IGVFDS2697OVVG
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR1-Rep1_S2
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR2-Rep1_S8
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR3-Rep1_S56
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR4-Rep1_S62
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci