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3 items
IGVFDS7833JNUC
  • jesse-engreitz:auxiliary_sortparams_CRUDO_FAM3C_Auxin0hrs-FF1
cell sorting
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin C for 35-45% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin D for 55-65% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, Homo sapiens (male) HCT116 cell line (sorting details: FACS bin F for 80-100% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
IGVFDS6449NYRM
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR1-Rep1_S110
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR2-Rep1_S116
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR3-Rep1_S164
  • jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR4-Rep1_S170
gRNA sequencing
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
revoked
gRNA sequencing
IGVFDS6704KMTH
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR1-Rep1_S110
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR2-Rep1_S116
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR3-Rep1_S164
  • jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR4-Rep1_S170
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci