in vitro CRISPR screen using single-cell RNA-seq

reads, seqspec

fastq, yaml

scCRISPR screen

Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

in vitro CRISPR screen using single-cell RNA-seq

reads, seqspec

fastq, yaml

scCRISPR screen

Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

in vitro CRISPR screen using single-cell RNA-seq

reads, seqspec

fastq, yaml

scCRISPR screen

Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

in vitro CRISPR screen using single-cell RNA-seq

reads, seqspec

fastq, yaml

scCRISPR screen

Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci