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/analysis-sets/IGVFDS7944XZYE/
4 items
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes