in vitro CRISPR screen using single-cell RNA-seq

reads

fastq

Perturb-seq

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW423) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

in vitro CRISPR screen using single-cell RNA-seq

reads

fastq

Perturb-seq

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW424) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

in vitro CRISPR screen using single-cell RNA-seq

reads

fastq

Perturb-seq

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW422) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

in vitro CRISPR screen using single-cell RNA-seq

reads

fastq

Perturb-seq

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW421) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci