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6 items
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
in vitro CRISPR screen using flow cytometry
reads
fastq
CRISPR FACS screen
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA