in vitro CRISPR screen using single-cell RNA-seq

reads

fastq

Perturb-seq

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

in vitro CRISPR screen using single-cell RNA-seq

reads

fastq

Perturb-seq

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW278) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

in vitro CRISPR screen using single-cell RNA-seq

reads

fastq

Perturb-seq

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW277) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

in vitro CRISPR screen using single-cell RNA-seq

reads

fastq

Perturb-seq

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci