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in vitro CRISPR screen using flow cytometry
25
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Variant-EFFECTS
25
Construct Library Data
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guide library
24
Readout Genes
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IL2RA
25
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Illumina NovaSeq X
25
input_for
/analysis-sets/IGVFDS1824XDMU/
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UUID
/measurement-sets/IGVFDS7995HXFV/
IGVFDS7995HXFV
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinC_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM4954YHLR/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
f89c61d9-a5b3-462e-a04b-5c39b2db99b4
/measurement-sets/IGVFDS2226SPYF/
IGVFDS2226SPYF
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinD_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM5709XAIS/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
e99c0c89-03a0-4772-b353-41f5a006d712
/measurement-sets/IGVFDS0414OFOP/
IGVFDS0414OFOP
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinD_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM9103RFFD/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
e24540ba-7cba-4dd3-90e1-466751b2ea78
/measurement-sets/IGVFDS6953SXZW/
IGVFDS6953SXZW
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep1-BinD_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM2690PEZO/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
d81ce09d-8886-4711-a9db-064cb0fce032
/measurement-sets/IGVFDS3851BRIR/
IGVFDS3851BRIR
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinC_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM8079DJVX/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
c0fad0f9-ca8f-4775-aecd-d2d55688b050
/measurement-sets/IGVFDS9863HSXN/
IGVFDS9863HSXN
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinA_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM6795SCHR/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
884a2802-3e38-4149-bf6b-5465eb62c3c4
/measurement-sets/IGVFDS3270YULL/
IGVFDS3270YULL
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinD_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM4553KVCE/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
825bb579-fbe0-4d9f-9326-0fd775cbd44e
/measurement-sets/IGVFDS9743TYOT/
IGVFDS9743TYOT
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep1-BinB_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM1469NQHM/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
7b03303b-c079-462c-98b9-d576a6db39e9
/measurement-sets/IGVFDS9762JBUG/
IGVFDS9762JBUG
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep1-BinC_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM4784GKZI/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
74e41340-0121-4c64-a2b8-c1865df1db1d
/measurement-sets/IGVFDS6874YIXA/
IGVFDS6874YIXA
jesse-engreitz:Jurkat_Stim-IL2RA_prom-NegControl_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM7816WZNZ/
preview
prime editing Variant-EFFECTS
Homo sapiens
6f4a08d5-029b-48de-bed2-ce5b23351385
/measurement-sets/IGVFDS2408JJSR/
IGVFDS2408JJSR
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinB_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM7352WPEI/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
6efead97-79fd-4dbf-821e-095291e63af4
/measurement-sets/IGVFDS9252NUTS/
IGVFDS9252NUTS
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinA_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM8750WCGQ/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
61ed9671-434f-4738-83e8-6eb86f63115d
/measurement-sets/IGVFDS0065BALX/
IGVFDS0065BALX
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-BinC_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM7206IJBO/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
5b680c76-da08-4c71-bde3-30be69d3a738
/measurement-sets/IGVFDS9444MPCG/
IGVFDS9444MPCG
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep1-BinA_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM7312DZBH/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
5a064bac-c577-4c9e-a4d5-4153f564957e
/measurement-sets/IGVFDS6913KQLF/
IGVFDS6913KQLF
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinA_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM2330LAXQ/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
58984b1c-7510-4980-8937-5305338ffa32
/measurement-sets/IGVFDS6025ASSH/
IGVFDS6025ASSH
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-BinA_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM5388SXBN/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
4dbb22aa-6cec-4450-b611-5fa0d92d18cf
/measurement-sets/IGVFDS9847QETL/
IGVFDS9847QETL
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinC_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM1409FVBR/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
469ec986-b776-4394-917c-c76c4e5bf1b8
/measurement-sets/IGVFDS7494CPQT/
IGVFDS7494CPQT
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-BinD_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM1871BLFB/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
43df0f25-db96-46c6-9544-101ad631b195
/measurement-sets/IGVFDS2419JBTK/
IGVFDS2419JBTK
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinB_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM9146UAUA/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
415d884b-e645-4b93-8626-7184722b0072
/measurement-sets/IGVFDS2584QAOI/
IGVFDS2584QAOI
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinC_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM9894RFCK/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
3fe3ae8b-128d-4019-98d9-3861c52f433d
/measurement-sets/IGVFDS4537KNTB/
IGVFDS4537KNTB
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinB_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM3690RUXJ/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
2afdb9b8-6029-417f-848a-0f5e28394b27
/measurement-sets/IGVFDS6967UVNI/
IGVFDS6967UVNI
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinA_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM9297KXBW/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
2a7b2fd3-0870-4ee3-ad51-fb830168d63a
/measurement-sets/IGVFDS2990MLAQ/
IGVFDS2990MLAQ
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-BinB_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM8345LMXC/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
29b220b7-94d8-40aa-91e4-e55f1c49f994
/measurement-sets/IGVFDS1434CFNM/
IGVFDS1434CFNM
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinD_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM9448WLVQ/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
1d33572f-86e9-411e-b332-aa5faf04e1e6
/measurement-sets/IGVFDS3202JLFH/
IGVFDS3202JLFH
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep3-BinB_AmpliconSequencing
IL2RA
/assay-terms/OBI_0003661/
/awards/HG011972/
/human-donors/IGVFDO1080XFGV/
experimental data
/labs/jesse-engreitz/
Variant-EFFECTS
https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1
/in-vitro-systems/IGVFSM4883HGPU/
preview
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Homo sapiens
0667f8ab-182a-4549-b8e2-b4e91eb052ad