Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library, Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library, Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library, Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 80-90% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library, Homo sapiens (male) Jurkat cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library, Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 35-45% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library, Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library, Homo sapiens (male) Jurkat cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
Homo sapiens Jurkat cell line, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library, sorted on expression of PPIF
prime editing Variant-EFFECTS targeting PPIF: variant effects