Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

low FACS signal CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA

Charles Gersbach, Duke

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