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Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

CRISPR FACS screen

guide (sgRNA) library targeting transcription start sites in many genes

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Description

Donors

Taxa

File Set Type

Functional Assay Mechanisms

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Sample Summary

Samples

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

FileSet

File Set

Sample

Files

Provenance

Quality

Object Type

Classification

Modification

Audit category: WARNING

Audit Category: Internal Action

Externally Hosted

control_for.@id

Report